Fig. 5

Electrically stimulated hNPCs with and without growth factors. (A-B) Diagram of experimental design and experimental groups- Effect of ES on hNPCs. Cells were expanded in neurospheres and then dissociated and cultured in differentiation medium for 2 days, then they were stimulated for 10 min on two consecutive days. After 4 days, the cells were fixed for immunoimaging analysis, (Image created with Biorender). (C) Representative fluorescence microscopy images of hNPCs when electrically stimulated and cultured without growth factors. (D) Represents the population of DAPI + cells without growth factors. (E) Represents the mean neurite length per neuron without growth factors. (F) Represents the population of MAP-2 + cells relative to the population of DAPI + cells without growth factors. (G) Represents the population of GAP-43 + cells relative to the population of DAPI + cells without growth factors. (H) Representative fluorescence microscopy images of hNPCs when electrically stimulated and cultured with growth factors. (I) Represents the population of DAPI + cells with growth factors. (J) Represents the mean neurite length per neuron with growth factors. (K) Represents the population of MAP-2 + cells relative to the population of DAPI + cells with growth factors. (L) Represents the population of GAP-43 + cells relative to the population of DAPI + cells with growth factors. ***p value < 0,001; ****p value < 0,0001. Data are show as mean ± SEM. Scale bar: 50 μm